Flow Cytometry

Flow Cytometry is powerful technology that has revolutionized biomedical research and become an integral investigative tool for research across diverse disciplines. With the use of fluorochrome-conjugated molecules such as monoclonal antibodies that specifically bind to its target antigen, flow cytometry allows for the rapid and simultaneous analysis of multiple parameters on millions of individual cells in single-cell suspensions.

The Flow Cytometry Core at the TNPRC, housed within the Division of Immunology, provides analytical flow cytometry services and live cell sorting under enhanced Biosafety Level 2 (BSL2+) conditions. Instruments available include six analyzers ranging from 13-48 color acquisition and three sorters using 1-,2-,4-way, and plate sorting between 6-18 colors. Both conventional and spectral analyzers are available. We provide experimental and panel design consultation as a service. The core is heavily utilized and supports research projects for a significant number of core and affiliate scientists.

Flow Core personnel and independent investigators use sample acquisition and cell sorting at TNPRC. The core is heavily utilized and supports research projects of at least 25 TNPRC core scientists and more than 30 affiliate scientists.

The Flow Cytometry Core will continue to expand its capability to keep abreast with state-of-the-art technology and maintain its high standard of service.

New users interacting with the flow core in any capacity (such as preparing samples or analyzing data) should book Flow Core Submission and Scheduling training for orientation via this scheduling link. After attending this training they may get access to Flowcytom data server, Flowjo license, Flow Cytometry listserv, recorded seminars, quality control records, and much more.

Acknowledgments

When utilizing research resources made possible by the TNPRC Flow Cytometry Core, please cite RRID: SCR_024611. For additional information on acknowledging Tulane National Primate Research Center resources, please see here.

Resources

BD LSRII 3-Laser
BD LSRFortessa 5-Laser
BD FACSSymphony A5 5-Laser
Miltenyi MACSQuant 16 3-Laser
Miltenyi MACSQuant 16 3-Laser

BD FACSSymphony A5 SE 5-Laser

BD FACS AriaFusion SORP 5-Laser
BD FACS Melody
Miltenyi Quant Tyto

iMac
MacBook Pro
Both are available to book via LabArchives Scheduler

Legendplex (bead based cytokine quantification using Biolegend Kit)
Absolute Count (volumetric CD45, CD3, CD4, CD8 whole blood quantification)
Consulting for high parameter panel design and in depth troubleshooting

FCC Policies (requires SSO login)
Self-Acquisition Terms of Service (available upon request)

Training is provided to access FCC. The entire process is outlined in User Training SOP (avaliable upon request)
Book New User Sample Submission and Scheduling Training. This training is mandatory to complete for all users interacting with the Flow Cytometry Core.
Self-acquisition Training is outlined in the User Training SOP (avaliable upon request).
Training Package - Self-acquisition Classroom to start self-acquisition training in the Training Package. Must be completed within 3 months of classroom training date.
Self-sorting training is avaliable for FACS Melody. Please email mmostrom@tulane.edu to get started.

Operator acquisition is booked in the FlowCore Box folder under the instruments with available operator acquisition
Hybrid acquisition (operator and user) is booked in Box and Scheduler
Self-acquisition is booked in LabArchives Scheduler
Cell sorter bookings are available to book over email for project-based requests
Absolute counts are availiable to book over email upon request

Free discussion meetings are available with the Assistant Director. Book here.
Consultations to book beyond initial discussions are available for a fee. Book here.
Flow Cytometry Core is open 8AM-4PM for staff working hours
After-hours operation of cytometers by users is available for proficient users
Contact Amitinder Kaur, MD, MBBS, Director of the Flow Cytometry Core
Contact Matilda Moström, PhD, SCYM, Assistant Director of the Flow Cytometry Core
Contact Natalie Valencia, Lab Supervisor of the Flow Cytometry Core