Xuebin Qin, MD, PhD

My research focuses on defining the role of innate immunity including complement system and monocyte activation in the pathogenesis of human diseases, such as HIV infection, HIV-associated cardiovascular diseases, and on developing a novel cell ablation research tool for broad scientific applications. I have extensive expertise in immunology, monocyte & macrophage biology, complement, HIV-1 therapy, atherosclerosis, and cancer biology. One of my research focuses has been to identify novel approach targeting complement system for HIV-1 therapy.

Another portion of my research is to investigate HIV-associated cardiovascular diseases, such as atherosclerosis. My major contributions to the fields include: 1) generation a novel and potent anti-MAC regulator CD59 inhibitor, a potential therapeutic candidate for control HIV-1 infections. 2) generation of the novel mouse model for HIV-associated atherosclerossi, 3) establishment of the causative role of MAC in atherosclerosis, 4) documentation of the inhibition of MAC with a targeted complement inhibitor as a potential novel approach for the treatment of atherosclerosis-associated complications, and 5) generation of a human CD59 mediated novel cell ablation for dissecting immune cell functionality and differentiation.

Research Interests

Therapeutic anti-hCD59 inhibitor for HIV and Ab-based cancer therapy

Intermedilysin (ILY), a cytolytic pore-forming toxin secreted by Streptococcus intermedius, lyses only human cells due to its receptor specificity for hCD59. Domain 4 of ILY binds to the AA42-58 region in hCD59, which also participates in the binding to C8 and C9. I demonstrated that the recombinant protein (114AA) derived from the ILY domain 4 (rlLYd4) specifically blocks hCD59 function, potentiates the antibody-mediated CDC effect on cancer cells and inhibits human xenograft tumor growth. It also potentiates endogenous anti-HIV antibody-mediated complement-dependent virolysis. The objective of this project is to develop the anti-hCD59 inhibitor for cancer and HIV therapy.

Universal rapid cell ablation model

We generated a universal rapid cell ablation model. We tested this ablation technique in various disease models and found it valuable for the study of cellular functionalities, tissue injury and regeneration, and neural injury. Currently, multiple investigators in USA are exploring the use of our model system in numerous areas. My lab is utilizing this tool to investigate the circulating and resident renal macrophage development, trafficking and gene expression, and study the mechanism underlying endothelial cell regeneration. I are collaborating with the faculties in the primate center to identify specific immune cells as additional latent reservoirs for HIV-infection in non-human primate model.

HIV-1-associated cardiovascular diseases (CVD)

HIV infection is associated with an increased risk of cardiovascular disease (CVD). Further study of the cellular and molecular mechanisms underlying HIV-1-associated CVD helps better develop and design the novel therapeutic strategies for atherosclerosis-associated CVD. However, the mechanisms underlying HIV-1-associated atherosclerosis have not been fully elucidated experimentally. This is mainly due to lack of the mouse model. I have generated and characterized a new mouse model for HIV-1-associated atherosclerosis. Currently, we are utilizing clinical samples and animal models (both mice and non-human primates) to investigate the atherogenic role of caspase-1 pathway activation and tryptophan catabolism via the kynurenine pathway in HIV-associated CVD and test the novel therapeutics for treatment of the disease.